RECONSTITUTION OF ALLOPHYCOCYANIN FROM Mastigocladus laminosus WITH ISOLATED LINKER POLYPEPTIDE

The core linker polypeptide Lc 8.9 was isolated from Mastigocladus laminosus and purified on a preparative scale. A method for the reconstitution of allophycocyanin (AP)—linker complexes from isolated polypeptides was developed. The complex (αAP(βAP)3 Lc 8.9 was reconstituted and compared to (αAPβAP) and (αAPβAP)3 by sucrose density gradient ultracentrifugation, absorption, fluorescence emission and circular dichroism spectroscopy. Differences in the spectra of reconstituted and of directly isolated AP complexes are discussed

Computational and theoretical aspects of a grain-boundary model at finite deformations

A model to describe the role of grain boundaries in the overall response of a polycrystalline material at small length scales subject to finite deformations is presented. Three alternative thermodynamically consistent plastic flow relations on the grain boundary are derived and compared using a series of numerical experiments. The numerical model is obtained by approximating the governing relations using the finite element method. In addition, the infinitesimal and finite deformation theories are compared, and the limitations of the former made clear

The Research Data Centre of the Centre for European Economic Research (ZEW-FDZ)

Das Zentrum für Europäische Wirtschaftsforschung (ZEW) in Mannheim stellt eine Reihe seiner Forschungsdatensätze externen Wissenschaftlern zur Verfügung. Das ZEW folgt damit den Empfehlungen der „Kommission zur Verbesserung der informationellen Infrastruktur zwischen Wissenschaft und Statistik“ und unterstützt so die Weiterentwicklung der empirischen Wirtschafts- und Sozialforschung. Im November 2012 ist das ZEW-FDZ vom Rat für Sozial und Wirtschaftsdaten (RatSWD) akkreditiert worden. Bei den im ZEW-FDZ angebotenen ZEW Forschungsdaten handelt es sich um Mikrodaten von ZEW Unternehmensbefragungen, ZEW Expertenbefragungen oder ZEW Personenbefragungen. Die Daten einiger ZEW-Befragungen werden externen Wissenschaftlern in einer faktisch anonymisierten Form als Scientific-Use-Files zur Verfügung gestellt. Neben der Nutzung der Scientific-Use-Files können externe Forscher einen Antrag auf Nutzung von formal anonymisierten ZEW-Daten stellen, mit denen sie in den FDZ-Räumen des ZEW arbeiten dürfen. Formal anonymisiert bedeutet, dass die Datensätze weder Namen noch Adressen, aber ansonsten alle Originalangaben der Befragten enthalten. Die formal anonymisierten Daten befinden sich dazu auf einem Stand-alone-Rechner ohne Netzwerkanbindung, von dem keine Daten heruntergeladen werden können. Für das Mannheimer Innovationspanel (MIP) wurden zudem absolut anonymisierte Education-Use-Files für die Nutzung in der Lehre erstellt. Das ZEW-FDZ schließt an die bisherige Praxis des ZEW an, eigene Erhebungsdaten an externe Wissenschaftler weiterzugeben. Vor dem Start des ZEW-FDZ im Januar 2013, stellte das ZEW etwa 280 Wissenschaftlern vom ZEW erhobene Forschungsdaten zur Verfügung; die meisten sind Nutzer des Mannheimer Innovationspanels (250). Das ZEW wird sein Datenangebot für externe Wissenschaftler laufend erweitern. Dies gilt auch für Daten zukünftiger Erhebunge

Computational and theoretical aspects of a grain-boundary model that accounts for grain misorientation and grain-boundary orientation

A detailed theoretical and numerical investigation of the infinitesimal single-crystal gradient plasticity and grain-boundary theory of Gurtin (2008) "A theory of grain boundaries that accounts automatically for grain misorientation and grain-boundary orientation". Journal of the Mechanics and Physics of Solids 56 (2), 640-662, is performed. The governing equations and flow laws are recast in variational form. The associated incremental problem is formulated in minimization form and provides the basis for the subsequent finite element formulation. Various choices of the kinematic measure used to characterize the ability of the grain boundary to impede the flow of dislocations are compared. An alternative measure is also suggested. A series of three-dimensional numerical examples serve to elucidate the theory

Cutting Through the Online Review Jungle — Investigating Selective eWOM Processing

Consumers frequently rely on online reviews, a prominent form of electronic word-of-mouth (eWOM), before making a purchase decision. However, consumers are usually confronted with hundreds of reviews for a single product or service, as well as rich information cues on online review websites (review texts, helpfulness ratings, author information, etc.). In turn, consumers face more information cues on online review websites than they can or want to process, and are likely to proceed selectively. This paper investigates selective processing of such eWOM information cues. Results of Study 1, an exploratory study using verbal protocols, confirm that consumers display selective eWOM processing patterns and are able to articulate them. Study 2 develops and applies a measurement instrument to capture these patterns. A subsequent cluster analysis on members of a large-scale online panel (N = 2,295) indicates five prominent eWOM processing types, termed “The Efficients”, “The Meticulous”, “The Quality-Evaluators”, “The Cautious Critics”, and “The Swift Pessimists”. Insights of this research can help firms to better understand consumers' eWOM processing and improve the user-friendliness of online review websites

The Six Frigates: The Epic History of the Founding of the U.S. Navy

Many books have been written about the history of the American navy, but this one is of particular excellence. While truly a scholarly work, this book contains many attributes of a historical novel. Any reader with an interest in either the U.S. Navy or early American history will find it hard to put down. Toll begins his story with a review of the Continental navy and its limited value during the American Revolution, then moves seamlessly into the post- revolutionary period. America’s colonial experience and the needs of the newly formed nation had a direct effect on the founding of a navy

Configurable Process Models as a Basis for Reference Modeling

Off-the-shelf packages such as SAP need to be configured to suit the requirements of an organization. Reference models support the configuration of these systems. Existing reference models use rather traditional languages. For example, the SAP reference model uses Eventdriven Process Chains (EPCs). Unfortunately, traditional languages like EPCs do not capture the configuration-aspects well. Consider for example the concept of "choice" in the control-flow perspective. Although any process modeling language, including EPCs, offers a choice construct (e.g., the XOR connector in EPCs), a single construct will not be able to capture the time dimension, scope, and impact of a decision. Some decisions are taken at run-time for a single case while other decisions are taken at build-time impacting a whole organization and all current and future cases. This position paper discusses the need for configurable process models as a basic building block for reference modeling. The focus is on the control-flow perspective. © Springer-Verlag Berlin Heidelberg 2006

Robust Flows over Time: Models and Complexity Results

We study dynamic network flows with uncertain input data under a robust optimization perspective. In the dynamic maximum flow problem, the goal is to maximize the flow reaching the sink within a given time horizon $T$, while flow requires a certain travel time to traverse an edge. In our setting, we account for uncertain travel times of flow. We investigate maximum flows over time under the assumption that at most $\Gamma$ travel times may be prolonged simultaneously due to delay. We develop and study a mathematical model for this problem. As the dynamic robust flow problem generalizes the static version, it is NP-hard to compute an optimal flow. However, our dynamic version is considerably more complex than the static version. We show that it is NP-hard to verify feasibility of a given candidate solution. Furthermore, we investigate temporally repeated flows and show that in contrast to the non-robust case (that is, without uncertainties) they no longer provide optimal solutions for the robust problem, but rather yield a worst case optimality gap of at least $T$. We finally show that the optimality gap is at most $O(\eta k \log T)$, where $\eta$ and $k$ are newly introduced instance characteristics and provide a matching lower bound instance with optimality gap $\Omega(\log T)$ and $\eta = k = 1$. The results obtained in this paper yield a first step towards understanding robust dynamic flow problems with uncertain travel times